Drd1 ELISA Kit (Rat)
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Drd1 ELISA Kit (Rat)
Gene Name :
Dopamine receptor D1Gene Aliases :
Dopamine D1 receptor.Gene ID :
24316Accession Number :
NP_036678.3Reactivity :
Rat, Rattus norvegicusTarget :
D (1A) dopamine receptorType :
ELISA KitApplications :
Enzyme-linked Immunosorbent assay-SandwichAssay Principle :
Aviva Systems Biology Drd1 ELISA Kit (Rat) (OKEH04821) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Drd1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Drd1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Drd1 captured in well.Assay Protocol :
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) ProtocolSample Type :
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.Detection Range :
0.312-20ng/mLSensitivity :
0.183 ng/mLReconstitution :
Store as indicated in product manual.Molecular Weight :
49 kDaSpecificity :
Natural and recombinant Rat D (1A) dopamine receptorProtein Length :
446NCBI Gene Symbol :
DRD1Protein Name :
P18901Gene Name URL :
DRD1CAS Number :
7732-18-5Nucleotide Accession Number :
NM_012546.3

