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KLK1 ELISA Kit (Mouse)

CAT: 0247-OKCD06675-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD06675-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Kallikrein 6 (KLK6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Kallikrein 6 (KLK6) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Kallikrein related-peptidase 6
Gene Aliases
AI849898; B; brain serine protease; BSP; Bssp; kallikrein 29; kallikrein 6; kallikrein-6; Klk2; Klk29; MSP; myelencephalon-specific protease; myofibril-bound protease; ne; neurosin; P; Pr; protease M; protease, serine, 18; protease, serine, 9; Prss18; Prss9.
Gene ID
19144
Accession Number
NP_001307260.1
Reactivity
Mouse|Mus musculus
Target
Kallikrein 6, isoform CRA_a
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallikrein 6 (KLK6) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kallikrein 6 (KLK6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kallikrein 6 (KLK6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kallikrein 6 (KLK6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, cerebrospinal fluid, breast milk and other biological fluids
Detection Range
31.2-2,000pg/mL
Sensitivity
< 13.4pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount KLK1 Microplate 96 Wells (12 x 8 Well strips) KLK1 Lyophilized Standard 2 100X KLK1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount KLK1 Microplate 96 Wells (12 x 8 Well strips) KLK1 Microplate 96 Wells (12 x 8 Well strips) KLK1 Lyophilized Standard 2 KLK1 Lyophilized Standard 2 100X KLK1 HRP-Detector Anitbody 1 x 120 uL 100X KLK1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
Klk6
Protein Name
Kallikrein 6, isoform CRA_a
Gene Name URL
Klk6
Nucleotide Accession Number
NM_001320331.1

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