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FGF3 ELISA Kit (Rat)

CAT: 0247-OKCD04484-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD04484-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Fibroblast Growth Factor 3 (FGF3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Fibroblast Growth Factor 3 (FGF3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Fibroblast growth factor 3
Gene Aliases
Fgf5c; fibroblast growth factor 3; fibroblast growth factor 5c; Int2; interacting gene 2.
Gene ID
170633
Accession Number
NP_570830.1
Reactivity
Rat|Rattus norvegicus
Target
Member of the fibroblast growth factor (FGF) family; implicated in activities including embryonic development, cell growth, morphogenesis, tissue growth, and tumor growth and invasion [RGD, Feb 2006]
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 3 (FGF3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 3 (FGF3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 3 (FGF3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibroblast Growth Factor 3 (FGF3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids.
Detection Range
15.62-1000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 99-105 102 EDTA Plasma (n=5) 84-91 87 Heparin Plasma (n=5) 83-96 93 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 99-105 102 Serum (n=5) 99-105 102 EDTA Plasma (n=5) 84-91 87 EDTA Plasma (n=5) 84-91 87 Heparin Plasma (n=5) 83-96 93 Heparin Plasma (n=5) 83-96 93
Sensitivity
5.8 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Fgf11 Microplate 96 Wells (12 x 8 Well strips) Fgf11 Lyophilized Standard 2 100X Fgf11 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Fgf11 Microplate 96 Wells (12 x 8 Well strips) Fgf11 Microplate 96 Wells (12 x 8 Well strips) Fgf11 Lyophilized Standard 2 Fgf11 Lyophilized Standard 2 100X Fgf11 HRP-Detector Anitbody 1 x 120 uL 100X Fgf11 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
Fgf3
Protein Name
Fibroblast growth factor
Gene Name URL
Fgf3
Nucleotide Accession Number
NM_130817.1

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