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VCC1 ELISA Kit (Mouse)

CAT: 0247-OKCD04477-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD04477-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level VEGF Co Regulated Chemokine 1 (VCC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level VEGF Co Regulated Chemokine 1 (VCC1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Chemokine (C-X-C motif) ligand 17
Gene Aliases
13.6 kDa protein;13.6-kDa protein;6-Cys CXCL17; C-X-C motif chemokine 17; Vcc1; VCC-1; VEGF coregulated chemokine 1; VEGF co-regulated chemokine 1.
Gene ID
232983
Accession Number
NP_705804.2
Reactivity
Mouse|Mus musculus
Target
Chemokine that acts as chemoattractant for monocytes, macrophages and dendritic cells (PubMed:24973458) . Plays a role in angiogenesis and possibly in the development of tumors (PubMed:16989774) . Acts as an anti-inflammatory in the stomach. May play a role in the innate defense against infections. Activates the C-X-C chemokine receptor GPR35 to induce a rapid and transient rise in the level of intracellular calcium ions.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGF Co Regulated Chemokine 1 (VCC1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VEGF Co Regulated Chemokine 1 (VCC1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VEGF Co Regulated Chemokine 1 (VCC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VEGF Co Regulated Chemokine 1 (VCC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
15.6-1,000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 96-103 99 EDTA Plasma (n=5) 90-97 93 Heparin Plasma (n=5) 92-103 96 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 96-103 99 Serum (n=5) 96-103 99 EDTA Plasma (n=5) 90-97 93 EDTA Plasma (n=5) 90-97 93 Heparin Plasma (n=5) 92-103 96 Heparin Plasma (n=5) 92-103 96
Sensitivity
6.1 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount CER Microplate 96 Wells (12 x 8 Well strips) CER Lyophilized Standard 2 100X CER HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount CER Microplate 96 Wells (12 x 8 Well strips) CER Microplate 96 Wells (12 x 8 Well strips) CER Lyophilized Standard 2 CER Lyophilized Standard 2 100X CER HRP-Detector Anitbody 1 x 120 uL 100X CER HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL
NCBI Gene Symbol
Cxcl17
Protein Name
C-X-C motif chemokine 17
Gene Name URL
Cxcl17
Nucleotide Accession Number
NM_153576.2

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