CLEC11A ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level C-Type Lectin Domain Family 11, Member A (CLEC11A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level C-Type Lectin Domain Family 11, Member A (CLEC11A) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

7732-18-5

C-type lectin domain containing 11A

CLECSF3; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 3; C-type lectin domain family 11 member A; C-type lectin superfamily member 3; LSLCL; lymphocyte secreted C-type lectin; lymphocyte secreted long form of C-type lectin; osteolectin; P47; SCGF; stem cell growth factor; stem cell growth factor; lymphocyte secreted C-type lectin.

6320

NP_002966.1

Homo sapiens|Human

Promotes osteogenesis by stimulating the differentiation of mesenchymal progenitors into mature osteoblasts (PubMed:27976999) . Important for repair and maintenance of adult bone (By similarity) .

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Lectin Domain Family 11, Member A (CLEC11A) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Type Lectin Domain Family 11, Member A (CLEC11A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Lectin Domain Family 11, Member A (CLEC11A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C-Type Lectin Domain Family 11, Member A (CLEC11A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, plasma, tissue homogenates and other biological fluids

0.94-60ng/mL

Matrix Recovery range (%) Average (%) Serum (n=5) 85-99 95 EDTA Plasma (n=5) 93-101 97 Heparin Plasma (n=5) 93-101 96 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 85-99 95 Serum (n=5) 85-99 95 EDTA Plasma (n=5) 93-101 97 EDTA Plasma (n=5) 93-101 97 Heparin Plasma (n=5) 93-101 96 Heparin Plasma (n=5) 93-101 96

0.39 ng/mL

2°C to 8°C|-20°C

Component Amount CLEC11A Microplate 96 Wells (12 x 8 Well strips) CLEC11A Lyophilized Standard 2 100X CLEC11A HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount CLEC11A Microplate 96 Wells (12 x 8 Well strips) CLEC11A Microplate 96 Wells (12 x 8 Well strips) CLEC11A Lyophilized Standard 2 CLEC11A Lyophilized Standard 2 100X CLEC11A HRP-Detector Anitbody 1 x 120 uL 100X CLEC11A HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

CLEC11A

C-type lectin domain family 11 member A

CLEC11A