XBP1 Chemi-Luminescent ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level X-Box Binding Protein 1 (XBP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level X-Box Binding Protein 1 (XBP1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  X-box binding protein 1

• Gene Aliases:

  D11Ertd39; D11Ertd39e; Tax-responsive element-binding protein 5; tax-responsive element-binding protein 5 homolog; TREB; TREB-; TREB5; TREB-5; X-box-binding protein 1; XBP-; XBP-1.

• Gene ID:

  22433

• Accession Number:

  NP_001258659

• Reactivity:

  Mouse|Mus musculus

• Target:

  Functions as a transcription factor during endoplasmic reticulum stress by regulating the unfolded protein response (UPR) . Required for cardiac myogenesis and hepatogenesis during embryonic development and the development of secretory tissues such as exocrine pancreas and salivary gland (PubMed:10425189, PubMed:10652269, PubMed:16362047, PubMed:17612490) . Involved in differentiation of B lymphocytes to plasma cells and production of immunoglobulins. Modulates the cellular response to ER stress in a PIK3R-dependent manner. Binds to the cis-acting X box present in the promoter regions of major histocompatibility complex class II genes (By similarity) . Involved in VEGF-induced endothelial cell (EC) proliferation and retinal blood vessel formation during embryonic development but also for angiogenesis in adult tissues under ischemic conditions (PubMed:23529610) . Functions also as a major regulator of the UPR in obesity-induced insulin resistance and type 2 diabetes for the management of obesity and diabetes prevention (PubMed:15486293) .

• Type:

  ELISA Kit

• Applications:

  Chemiluminescent immunoassay

• Detection Method:

  Chemiluminescent

• Assay Principle:

  The microplate provided in this kit has been pre-coated with an antibody specific to X-Box Binding Protein 1 (XBP1) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to X-Box Binding Protein 1 (XBP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the X-Box Binding Protein 1 (XBP1) level in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Tissue homogenates and other biological fluids

• Detection Range:

  13.7-10,000pg/mL

• Sensitivity:

  0.156 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Xbp1 Microplate 96 Wells (12 x 8 Well strips) Xbp1 Lyophilized Standard 2 100X Xbp1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Xbp1 Microplate 96 Wells (12 x 8 Well strips) Xbp1 Microplate 96 Wells (12 x 8 Well strips) Xbp1 Lyophilized Standard 2 Xbp1 Lyophilized Standard 2 100X Xbp1 Biotinylated Detector Anitbody 1 x 120 uL 100X Xbp1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL

• NCBI Gene Symbol:

  Xbp1

• Protein Name:

  X-box-binding protein 1

• Gene Name URL:

  Xbp1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001271730