EIF2AK3 ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Eukaryotic translation initiation factor 2 alpha kinase 3

• Gene Aliases:

  Eukaryotic translation initiation factor 2-alpha kinase 3; pancreatic eIF2-alpha kinase; PE; Pek; Perk; PRKR-like endoplasmic reticulum kinase.

• Gene ID:

  13666

• Accession Number:

  NP_001300847.1

• Reactivity:

  Mouse|Mus musculus

• Target:

  Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability (PubMed:11106749) . Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:23921556) . Serves as a critical effector of unfolded protein response (UPR) -induced G1 growth arrest due to the loss of cyclin-D1 (CCND1) (PubMed:11035797) . Involved in control of mitochondrial morphology and function (PubMed:23921556) .

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Tissue homogenates and other biological fluids

• Detection Range:

  0.312-20ng/mL

• Sensitivity:

  0.108 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-EIF2AK3 Microplate 96 Wells (12 x 8 Well strips) EIF2AK3 Lyophilized Standard 2 100X Biotinylated EIF2AK3 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-EIF2AK3 Microplate 96 Wells (12 x 8 Well strips) Anti-EIF2AK3 Microplate 96 Wells (12 x 8 Well strips) EIF2AK3 Lyophilized Standard 2 EIF2AK3 Lyophilized Standard 2 100X Biotinylated EIF2AK3 Detector Antibody 1 x 120 uL 100X Biotinylated EIF2AK3 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) . No significant cross-reactivity or interference between Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) and analogues was observed.

• Protein Length:

  1114

• NCBI Gene Symbol:

  Eif2ak3

• Protein Name:

  Eukaryotic translation initiation factor 2-alpha kinase 3

• Gene Name URL:

  Eif2ak3

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001313918.1