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ALDOB ELISA Kit (Human)

CAT: 0247-OKCD02355-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02355-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Aldolase B, Fructose Bisphosphate (ALDOB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Aldolase B, Fructose Bisphosphate (ALDOB) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Aldolase, fructose-bisphosphate B
Gene Aliases
ALDB; ALDO2; aldolase 2; aldolase B, fructose-bisphosphatase; aldolase B, fructose-bisphosphate; fructose-bisphosphate aldolase B; liver-type aldolase.
Gene ID
229
Accession Number
NP_000026.2
Reactivity
Homo sapiens|Human
Target
Fructose-bisphosphate aldolase B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldolase B, Fructose Bisphosphate (ALDOB) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldolase B, Fructose Bisphosphate (ALDOB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldolase B, Fructose Bisphosphate (ALDOB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aldolase B, Fructose Bisphosphate (ALDOB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
2.5-160ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 79-89 83 EDTA Plasma (n=5) 92-101 96 Heparin Plasma (n=5) 78-93 85 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 79-89 83 Serum (n=5) 79-89 83 EDTA Plasma (n=5) 92-101 96 EDTA Plasma (n=5) 92-101 96 Heparin Plasma (n=5) 78-93 85 Heparin Plasma (n=5) 78-93 85
Sensitivity
0.11 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-ALDOB Microplate 96 Wells (12 x 8 Well strips) ALDOB Lyophilized Standard 2 100X Biotinylated ALDOB Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-ALDOB Microplate 96 Wells (12 x 8 Well strips) Anti-ALDOB Microplate 96 Wells (12 x 8 Well strips) ALDOB Lyophilized Standard 2 ALDOB Lyophilized Standard 2 100X Biotinylated ALDOB Detector Antibody 1 x 120 uL 100X Biotinylated ALDOB Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Aldolase B, Fructose Bisphosphate (ALDOB) . No significant cross-reactivity or interference between Aldolase B, Fructose Bisphosphate (ALDOB) and analogues was observed.
Protein Length
364
NCBI Gene Symbol
ALDOB
Protein Name
Fructose-bisphosphate aldolase B
Gene Name URL
ALDOB
Nucleotide Accession Number
NM_000035.3

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