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Levonorgestrel ELISA Kit

CAT: 0247-OKCD02273-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02273-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Levonorgestrel (LNG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Levonorgestrel (LNG) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Aliases
Levonorgestrel; Norgestrel; 797-63-7; Microval; Postinor
Reactivity
All Species
Target
Levonorgestrel is a synthetic progestational hormone with actions similar to those of PROGESTERONE and about twice as potent as its racemic or (+-) -isomer (NORGESTREL) . It is used for contraception, control of menstrual disorders, and treatment of endometriosis.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Detection Method
Colorimetric
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Levonorgestrel (LNG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Levonorgestrel (LNG) and unlabeled Levonorgestrel (LNG) (Standards or samples) with the pre-coated antibody specific to Levonorgestrel (LNG) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Levonorgestrel (LNG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Levonorgestrel (LNG) in the sample.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.23-100ng/mL
Recovery
Matrix Recovery Range (%) Average (%) serum (n=5) 92-99 96 EDTA plasma (n=5) 92-101 96 heparin plasma (n=5) 83-101 91 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 92-99 96 serum (n=5) 92-99 96 EDTA plasma (n=5) 92-101 96 EDTA plasma (n=5) 92-101 96 heparin plasma (n=5) 83-101 91 heparin plasma (n=5) 83-101 91
Sensitivity
0.53 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Levonorgestrel Microplate 96 Wells (12 x 8 Well Strips) Levonorgestrel Lyophilized Standard 2 100X Levonorgestrel Biotin Complex 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Levonorgestrel Microplate 96 Wells (12 x 8 Well Strips) Anti-Levonorgestrel Microplate 96 Wells (12 x 8 Well Strips) Levonorgestrel Lyophilized Standard 2 Levonorgestrel Lyophilized Standard 2 100X Levonorgestrel Biotin Complex 120 uL 100X Levonorgestrel Biotin Complex 120 uL 100X Avidin/HRP Conjugate 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
LNG
Protein Name
Levonorgestrel
Gene Name URL
LNG

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