Endomorphin 2 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Endomorphin 2 (EM2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Endomorphin 2 (EM2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

7732-18-5

Endomorphin 2; Endomorphin-2; Tyr-Pro-Phe-Phe-NH2

Human

ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

Colorimetric

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Endomorphin 2 (EM2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Endomorphin 2 (EM2) and unlabeled Endomorphin 2 (EM2) (Standards or samples) with the pre-coated antibody specific to Endomorphin 2 (EM2) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Endomorphin 2 (EM2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Endomorphin 2 (EM2) in the sample.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, plasma and other biological fluids.

3.70-300pg/mL

Matrix Recovery Range (%) Average (%) serum (n=5) 88-104 97 EDTA plasma (n=5) 91-103 101 heparin plasma (n=5) 84-98 95 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 88-104 97 serum (n=5) 88-104 97 EDTA plasma (n=5) 91-103 101 EDTA plasma (n=5) 91-103 101 heparin plasma (n=5) 84-98 95 heparin plasma (n=5) 84-98 95

1.56 pg/mL

2°C to 8°C|-20°C

Component Amount Anti-Endomorphin 2 Microplate 96 Wells (12 x 8 Well Strips) Endomorphin 2 Lyophilized Standard 2 100X Endomorphin 2 Biotin Complex 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Endomorphin 2 Microplate 96 Wells (12 x 8 Well Strips) Anti-Endomorphin 2 Microplate 96 Wells (12 x 8 Well Strips) Endomorphin 2 Lyophilized Standard 2 Endomorphin 2 Lyophilized Standard 2 100X Endomorphin 2 Biotin Complex 120 uL 100X Endomorphin 2 Biotin Complex 120 uL 100X Avidin/HRP Conjugate 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

EM2

Endomorphin 2