Products for Research Use Only
RegisterLogin

IGLL5 ELISA Kit (Human)

CAT: 0247-OKCD02120-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD02120-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Immunoglobulin Lambda Like Polypeptide 5 (IglL5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Immunoglobulin Lambda Like Polypeptide 5 (IglL5) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Immunoglobulin lambda like polypeptide 5
Gene Aliases
Anti HBs antibody light-chain Fab fragment; G lambda-1; germline immunoglobulin lambda 1; IGL; IGLV; immunoglobin anti-granzymeB light chain variable region; immunoglobulin lambda 1 light chain; immunoglobulin lambda 2 light chain; immunoglobulin lambda 3 light chain; immunoglobulin lambda light chain variable region; immunoglobulin lambda-3 surrogate light chain; immunoglobulin lambda-like polypeptide 5; immunoglobulin light chain variable region EM1-PPS-4-L1-1; omega light chain protein 14.1; VL-MAR.
Gene ID
100423062
Accession Number
NP_001171597.1
Reactivity
Homo sapiens|Human
Target
This gene encodes one of the immunoglobulin lambda-like polypeptides. It is located within the immunoglobulin lambda locus but it does not require somatic rearrangement for expression. The first exon of this gene is unrelated to immunoglobulin variable genes; the second and third exons are the immunoglobulin lambda joining 1 and the immunoglobulin lambda constant 1 gene segments. Alternative splicing results in multiple transcript variants.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin Lambda Like Polypeptide 5 (IglL5) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin Lambda Like Polypeptide 5 (IglL5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin Lambda Like Polypeptide 5 (IglL5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Immunoglobulin Lambda Like Polypeptide 5 (IglL5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.124 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-IGLL5 Microplate 96 Wells (12 x 8 Well Strips) IGLL5 Lyophilized Standard 2 x 20 ng 100X Biotinylated IGLL5 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-IGLL5 Microplate 96 Wells (12 x 8 Well Strips) Anti-IGLL5 Microplate 96 Wells (12 x 8 Well Strips) IGLL5 Lyophilized Standard 2 x 20 ng IGLL5 Lyophilized Standard 2 x 20 ng 100X Biotinylated IGLL5 Detector Antibody 120 uL 100X Biotinylated IGLL5 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBBQ8
Specificity
This assay has high sensitivity and excellent specificity for detection of Immunoglobulin Lambda Like Polypeptide 5 (IglL5) . No significant cross-reactivity or interference between Immunoglobulin Lambda Like Polypeptide 5 (IglL5) and analogues was observed.
Protein Length
214
NCBI Gene Symbol
IGLL5
Protein Name
Immunoglobulin lambda-like polypeptide 5
Gene Name URL
IGLL5
Nucleotide Accession Number
NM_001178126.1

Related Products

CatalogName
OPCA02975-100UGIGLL5 Recombinant Protein
OCOA20960-20UGIGLL5 Protein Lysate
OPCA02975-20UGIGLL5 Recombinant Protein
OAAB13993-400ULIGLL5 Antibody - C-terminal region
OPCA02975-500UGOPCA02975-500UG - IGLL5 Recombinant Protein
OKCD06003-96WKIT ELISA Kit (Human)
OKEH00203-96WKIT ELISA Kit (Human)
OKEH05298-96WWAS ELISA Kit (Human)
OKAN04574KIT ELISA Kit (Human) (OKAN04574)
OKCD06003KIT ELISA Kit (Human) (OKCD06003)

Alternative Products

CatalogName
E019632Human IglL5 ELISA Kit
EK246993Human IglL5 (Immunoglobulin Lambda Like Polypeptide 5) ELISA Kit
MBS2707349-01Human Immunoglobulin Lambda Like Polypeptide 5 (IglL5) ELISA Kit
MBS2905403-01Human IGLL5 / Immunoglobulin lambda-like polypeptide 5 ELISA Kit
ELI-12466hHuman Immunoglobulin lambda-like polypeptide 5, IGLL5 ELISA KIT
KBH21258GENLISA™ Human Immunoglobulin Lambda Like Polypeptide 5 (IglL5) ELISA
GTR18871878-01Human IglL5 (Immunoglobulin Lambda Like Polypeptide 5) Microsample ELISA Kit
366148Immunoglobulin Lambda Like Polypeptide 5 (IglL5) BioAssayTM ELISA Kit (Human)
P16890POTB7-IGLL5 (human)
GTR19319827-01IGLL5 siRNA (Human)

Popular Products