EDIL3 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

7732-18-5

EGF like repeats and discoidin domains 3

DEL1; developmental endothelial locus-1; developmentally-regulated endothelial cell locus 1 protein; EGF-like repeat and discoidin I-like domain-containing protein 3; EGF-like repeats and discoidin I-like domains 3; integrin-binding protein DEL1.

10085

NP_001265571.1

Homo sapiens|Human

Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development.

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Tissue homogenates, cell lysates and other biological fluids

0.312-20ng/mL

< 0.127 ng/mL

2°C to 8°C|-20°C

Component Amount Anti-EDIL3 Microplate 96 Wells (12 x 8 Well Strips) EDIL3 Lyophilized Standard 2 x 20 ng 100X Biotinylated EDIL3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-EDIL3 Microplate 96 Wells (12 x 8 Well Strips) Anti-EDIL3 Microplate 96 Wells (12 x 8 Well Strips) EDIL3 Lyophilized Standard 2 x 20 ng EDIL3 Lyophilized Standard 2 x 20 ng 100X Biotinylated EDIL3 Detector Antibody 120 uL 100X Biotinylated EDIL3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

Formerly GWB-KBBWH1

This assay has high sensitivity and excellent specificity for detection of EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3) . No significant cross-reactivity or interference between EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3) and analogues was observed.

480

EDIL3

EGF-like repeat and discoidin I-like domain-containing protein 3

EDIL3