COCH ELISA Kit (Human)

CAT: 0247-OKCD02001-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD02001-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cochlin (COCH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cochlin (COCH) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Cochlin
Gene Aliases
Coagulation factor C homolog, cochlin (Limulus polyphemus) ; COCH5B2; COCH-5B2; cochlin; DFNA9; DFNB110.
Gene ID
1690
Accession Number
NP_001128530.1
Reactivity
Homo sapiens|Human
Target
Plays a role in the control of cell shape and motility in the trabecular meshwork.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cochlin (COCH) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cochlin (COCH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cochlin (COCH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cochlin (COCH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.057 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-COCH Microplate 96 Wells (12 x 8 Well Strips) COCH Lyophilized Standard 2 x 10 ng 100X Biotinylated COCH Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-COCH Microplate 96 Wells (12 x 8 Well Strips) Anti-COCH Microplate 96 Wells (12 x 8 Well Strips) COCH Lyophilized Standard 2 x 10 ng COCH Lyophilized Standard 2 x 10 ng 100X Biotinylated COCH Detector Antibody 120 uL 100X Biotinylated COCH Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBOR3
Specificity
This assay has high sensitivity and excellent specificity for detection of Cochlin (COCH) . No significant cross-reactivity or interference between Cochlin (COCH) and analogues was observed.
Protein Length
550
NCBI Gene Symbol
COCH
Protein Name
Cochlin
Gene Name URL
COCH
Nucleotide Accession Number
NM_001135058.1

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