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PCSK2 ELISA Kit (Human)

CAT: 0247-OKCD01811-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01811-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Proprotein convertase subtilisin/kexin type 2
Gene Aliases
KEX2-like endoprotease 2; NEC 2; NEC2; NEC-2; neuroendocrine convertase 2; PC2; prohormone convertase 2; Proprotein convertase 2; SPC2.
Gene ID
5126
Accession Number
NP_001188457.1
Reactivity
Homo sapiens|Human
Target
Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.112 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-PCSK2 Microplate 96 Wells (12 x 8 Well Strips) PCSK2 Lyophilized Standard 2 x 20 ng 100X Biotinylated PCSK2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-PCSK2 Microplate 96 Wells (12 x 8 Well Strips) Anti-PCSK2 Microplate 96 Wells (12 x 8 Well Strips) PCSK2 Lyophilized Standard 2 x 20 ng PCSK2 Lyophilized Standard 2 x 20 ng 100X Biotinylated PCSK2 Detector Antibody 120 uL 100X Biotinylated PCSK2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBEY7
Specificity
This assay has high sensitivity and excellent specificity for detection of Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) . No significant cross-reactivity or interference between Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) and analogues was observed.
Protein Length
638
NCBI Gene Symbol
PCSK2
Protein Name
Neuroendocrine convertase 2
Gene Name URL
PCSK2
Nucleotide Accession Number
NM_001201528.1

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