OLR1 ELISA Kit (Rabbit)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

7732-18-5

Oxidized low density lipoprotein receptor 1

Lectin-like oxidized LDL receptor 1; lectin-like oxLDL receptor 1; lectin-type oxidized LDL receptor 1; LOX1; LOX-1; oxidized low density lipoprotein (lectin-like) receptor 1; oxidized low-density lipoprotein receptor 1; ox-LDL receptor 1.

100009322

NP_001076102.1

Oryctolagus cuniculus|Rabbit

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria (By similarity) .

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, plasma, tissue homogenates and other biological fluids

78-5,000pg/mL

Matrix Recovery Range (%) Average (%) Serum (n=5) 92-101 95 EDTA Plasma (n=5) 90-98 93 Heparin Plasma (n=5) 80-98 93 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum (n=5) 92-101 95 Serum (n=5) 92-101 95 EDTA Plasma (n=5) 90-98 93 EDTA Plasma (n=5) 90-98 93 Heparin Plasma (n=5) 80-98 93 Heparin Plasma (n=5) 80-98 93

< 24 pg/mL

2°C to 8°C|-20°C

Component Amount Anti-OLR1 Microplate 96 Wells (12 x 8 Well Strips) OLR1 Lyophilized Standard 2 x 5 ng 100X Biotinylated OLR1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-OLR1 Microplate 96 Wells (12 x 8 Well Strips) Anti-OLR1 Microplate 96 Wells (12 x 8 Well Strips) OLR1 Lyophilized Standard 2 x 5 ng OLR1 Lyophilized Standard 2 x 5 ng 100X Biotinylated OLR1 Detector Antibody 120 uL 100X Biotinylated OLR1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

Formerly GWB-KBBYH9

This assay has high sensitivity and excellent specificity for detection of Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) . No significant cross-reactivity or interference between Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) and analogues was observed.

274

OLR1

Oxidized low-density lipoprotein receptor 1

OLR1