Products for Research Use Only
RegisterLogin

ADIPOR2 ELISA Kit (Human)

CAT: 0247-OKCD01488-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD01488-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Adiponectin Receptor 2 (ADIPOR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Adiponectin Receptor 2 (ADIPOR2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Adiponectin receptor 2
Gene Aliases
ACDCR2; adiponectin receptor protein 2; PAQR2; progestin and adipoQ receptor family member 2; progestin and adipoQ receptor family member II.
Gene ID
79602
Accession Number
NP_078827.2
Reactivity
Homo sapiens|Human
Target
Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism (PubMed:12802337, PubMed:25855295) . Required for normal body fat and glucose homeostasis. ADIPOQ-binding activates a signaling cascade that leads to increased PPARA activity, and ultimately to increased fatty acid oxidation and glucose uptake. Has intermediate affinity for globular and full-length adiponectin. Required for normal revascularization after chronic ischemia caused by severing of blood vessels (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin Receptor 2 (ADIPOR2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adiponectin Receptor 2 (ADIPOR2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adiponectin Receptor 2 (ADIPOR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adiponectin Receptor 2 (ADIPOR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.78-50ng/mL
Sensitivity
< 0.27 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-ADIPOR2 Microplate 96 Wells (12 x 8 Well Strips) ADIPOR2 Lyophilized Standard 2 x 50 ng 100X Biotinylated ADIPOR2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-ADIPOR2 Microplate 96 Wells (12 x 8 Well Strips) Anti-ADIPOR2 Microplate 96 Wells (12 x 8 Well Strips) ADIPOR2 Lyophilized Standard 2 x 50 ng ADIPOR2 Lyophilized Standard 2 x 50 ng 100X Biotinylated ADIPOR2 Detector Antibody 120 uL 100X Biotinylated ADIPOR2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBVU9
Specificity
This assay has high sensitivity and excellent specificity for detection of Adiponectin Receptor 2 (ADIPOR2) . No significant cross-reactivity or interference between Adiponectin Receptor 2 (ADIPOR2) and analogues was observed.
Protein Length
386
NCBI Gene Symbol
ADIPOR2
Protein Name
Adiponectin receptor protein 2
Gene Name URL
ADIPOR2
Nucleotide Accession Number
NM_024551.2

Related Products

CatalogName
OKCD00786-96WADIPOR2 ELISA Kit (Rat)
OKCD00786ADIPOR2 ELISA Kit (Rat) (OKCD00786)
OACD01448-100ULADIPOR2 Antibody
OEEG01136-50UGADIPOR2 Peptide
OACD01448-1MLADIPOR2 Antibody
OACD01446-1MLADIPOR2 Antibody
OACD01446-100ULADIPOR2 Antibody
OPCD01134-10UGADIPOR2 Recombinant Protein
OPCD01134-50UGADIPOR2 Recombinant Protein
OPCD01134-200UGADIPOR2 Recombinant Protein

Alternative Products

CatalogName
EHA0532Human ADIPOR2 ELISA Kit
EF006574Human ADIPOR2 ELISA KIT
NBP2-66647Human AdipoR2 ELISA Kit (Chemiluminescence)
GTR18828534-01Human ADIPOR2 ELISA Kit (Ready to Use)
MBS8801957-01Human ADIPOR2 (Adiponectin Receptor 2) ELISA Kit
MBS3801733-01Human Adiponectin Receptor 2 (ADIPOR2) ELISA Kit
KBH20080GENLISA™ Human Adiponectin Receptor 2 (ADIPOR2) ELISA
B2977Adiponectin Receptor 2 (ADIPOR2) Human ELISA Kit
11427051ADIPOR2 Adenovirus (Human)
MBS8230260-01ADIPOR2 siRNA (Human)

Popular Products