Bmp2 ELISA Kit (Mouse)

CAT: 0247-OKCD01435-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD01435-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Bone morphogenetic protein 2
Gene Aliases
AI467020; Bmp; Bmp2a; bone morphogenetic protein 2; bone morphogenetic protein 2A.
Gene ID
12156
Accession Number
NP_031579.2
Reactivity
Mouse|Mus musculus
Target
Induces cartilage and bone formation. Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 2 (BMP2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 2 (BMP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 2 (BMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bone Morphogenetic Protein 2 (BMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
15.6-1,000pg/mL
Recovery
Matrix Recovery Range (%) Average (%) Serum (n=5) 87-97 91 EDTA Plasma (n=5) 94-105 98 Heparin Plasma (n=5) 93-103 96 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum (n=5) 87-97 91 Serum (n=5) 87-97 91 EDTA Plasma (n=5) 94-105 98 EDTA Plasma (n=5) 94-105 98 Heparin Plasma (n=5) 93-103 96 Heparin Plasma (n=5) 93-103 96
Sensitivity
< 3.3 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Bmp2 Microplate 96 Wells (12 x 8 Well Strips) Bmp2 Lyophilized Standard 2 x 500 pg 100X Biotinylated Bmp2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Bmp2 Microplate 96 Wells (12 x 8 Well Strips) Anti-Bmp2 Microplate 96 Wells (12 x 8 Well Strips) Bmp2 Lyophilized Standard 2 x 500 pg Bmp2 Lyophilized Standard 2 x 500 pg 100X Biotinylated Bmp2 Detector Antibody 120 uL 100X Biotinylated Bmp2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBUZ9
Specificity
This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 2 (BMP2) . No significant cross-reactivity or interference between Bone Morphogenetic Protein 2 (BMP2) and analogues was observed.
Protein Length
394
NCBI Gene Symbol
Bmp2
Protein Name
Bone morphogenetic protein 2
Gene Name URL
Bmp2
Nucleotide Accession Number
NM_007553.3

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