Bmp2 ELISA Kit (Dog)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 51 198 802 48 221 789 Standard deviation 2.19 10.09 34.48 2.54 12.15 32.34 CV (%) 4.3 4.1 4.3 5.3 5.5 4.1 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 51 198 802 48 221 789 Mean (pg/ml) 51 198 802 48 221 789 Standard deviation 2.19 10.09 34.48 2.54 12.15 32.34 Standard deviation 2.19 10.09 34.48 2.54 12.15 32.34 CV (%) 4.3 4.1 4.3 5.3 5.5 4.1 CV (%) 4.3 4.1 4.3 5.3 5.5 4.1

• Gene Name:

  Bone morphogenetic protein 2

• Gene Aliases:

  Bone morphogenetic protein 2; BMP-2; Bone morphogenetic protein 2A; BMP-2A; Bmp2; Bmp-2

• Gene ID:

  477162

• Accession Number:

  NP_031579.2

• Reactivity:

  Dog

• Immunogen:

  Expression system for standard: CHO; Immunogen sequence: Q283-R396

• Target:

  Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant Dog Canine BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's Canine BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-2 has been precoated onto 96-well plates. Standards (CHO, Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Canine BMP-2 amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Sandwich High Sensitivity ELISA kit for Quantitative Detection of Dog Canine BMP-2

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <2 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• NCBI Gene Symbol:

  BMP2

• Protein Name:

  Bone morphogenetic protein 2

• Gene Name URL:

  BMP2

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_007553.3