ACE ELISA Kit (Rabbit)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Angiotensin I converting enzyme

• Gene Aliases:

  ACE-T; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; angiotensin-converting enzyme; DCP1; dipeptidyl carboxypeptidase I; kininase II; peptidyl dipeptidase i.

• Gene ID:

  100009274

• Accession Number:

  NP_001075864.1

• Reactivity:

  Oryctolagus cuniculus|Rabbit

• Target:

  Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety (By similarity) .

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme (ACE) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme (ACE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin I Converting Enzyme (ACE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Angiotensin I Converting Enzyme (ACE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  0.312-20ng/mL

• Recovery:

  Matrix Recovery Range (%) Average (%) Serum (n=5) 82-104 97 EDTA Plasma (n=5) 92-105 99 Heparin Plasma (n=5) 88-96 91 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum (n=5) 82-104 97 Serum (n=5) 82-104 97 EDTA Plasma (n=5) 92-105 99 EDTA Plasma (n=5) 92-105 99 Heparin Plasma (n=5) 88-96 91 Heparin Plasma (n=5) 88-96 91

• Sensitivity:

  < 0.128 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-ACE Microplate 96 Wells (12 x 8 Well Strips) ACE Lyophilized Standard 2 x 20 ng 100X Biotinylated ACE Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-ACE Microplate 96 Wells (12 x 8 Well Strips) Anti-ACE Microplate 96 Wells (12 x 8 Well Strips) ACE Lyophilized Standard 2 x 20 ng ACE Lyophilized Standard 2 x 20 ng 100X Biotinylated ACE Detector Antibody 120 uL 100X Biotinylated ACE Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes:

  Formerly GWB-KBBVS9

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Angiotensin I Converting Enzyme (ACE) . No significant cross-reactivity or interference between Angiotensin I Converting Enzyme (ACE) and analogues was observed.

• Protein Length:

  1310

• NCBI Gene Symbol:

  ACE

• Protein Name:

  Angiotensin-converting enzyme

• Gene Name URL:

  ACE

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001082395.1