IFNB High Sensitivity ELISA Kit (Chicken)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level High Sensitive Interferon Beta (IFNb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level High Sensitive Interferon Beta (IFNb) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Interferon omega 1

• Gene Aliases:

  IFN_B; IFNB; IFN-beta; interferon type B; interferon, beta 1, fibroblast; interferon-beta.

• Gene ID:

  554219

• Accession Number:

  NP_001020007.1

• Reactivity:

  Chicken|Gallus gallus

• Target:

  Has antiviral activities.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Interferon Beta (IFNb) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Interferon Beta (IFNb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Interferon Beta (IFNb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Interferon Beta (IFNb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  3.12-200pg/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 87-105 99 EDTA Plasma (n=5) 78-99 86 Heparin Plasma (n=5) 82-90 85 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 87-105 99 Serum (n=5) 87-105 99 EDTA Plasma (n=5) 78-99 86 EDTA Plasma (n=5) 78-99 86 Heparin Plasma (n=5) 82-90 85 Heparin Plasma (n=5) 82-90 85

• Sensitivity:

  1.16 pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-IFNB Microplate 96 Wells (12 x 8 Well Strips) IFNB Lyophilized Standard 2 100X Biotinylated IFNB Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-IFNB Microplate 96 Wells (12 x 8 Well Strips) Anti-IFNB Microplate 96 Wells (12 x 8 Well Strips) IFNB Lyophilized Standard 2 IFNB Lyophilized Standard 2 100X Biotinylated IFNB Detector Antibody 120 uL 100X Biotinylated IFNB Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Interferon Beta (IFNb) . No significant cross-reactivity or interference between Interferon Beta (IFNb) and analogues was observed.

• NCBI Gene Symbol:

  IFNW1

• Protein Name:

  Interferon type B

• Gene Name URL:

  IFNW1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001024836.1