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Complement 1q Antibody ELISA Kit (Human)

CAT: 0247-OKCD01291-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01291-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Anti-Complement 1q Antibody (Anti-C1q) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Anti-Complement 1q Antibody (Anti-C1q) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Complement C1q A chain|complement C1q B chain|complement C1q C chain
Gene Aliases
C1Q-C; C1QG; complement C1q chain A; complement C1q chain B; complement C1q subcomponent subunit A; complement C1q subcomponent subunit B; complement C1q subcomponent subunit C; complement component 1, q subcomponent, A chain; complement component 1, q subcomponent, alpha polypeptide; complement component 1, q subcomponent, B chain; complement component 1, q subcomponent, beta polypeptide; complement component 1, q subcomponent, C chain; complement component 1, q subcomponent, gamma polypeptide; complement component C1q, A chain; complement component C1q, B chain; complement subcomponent C1q chain B.
Gene ID
712|713|714
Reactivity
Homo sapiens|Human
Target
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca (2+) -dependent C1r (2) C1s (2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Detection Method
Colorimetric
Assay Principle
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Complement 1q Antibody (Anti-C1q) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Complement 1q Antibody (Anti-C1q) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.56-100ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 78-103 89 EDTA Plasma (n=5) 78-101 82 Heparin Plasma (n=5) 91-98 95 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 78-103 89 Serum (n=5) 78-103 89 EDTA Plasma (n=5) 78-101 82 EDTA Plasma (n=5) 78-101 82 Heparin Plasma (n=5) 91-98 95 Heparin Plasma (n=5) 91-98 95
Sensitivity
0.58 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Complement 1q antibody Microplate 96 Wells (12 x 8 Well strips) Complement 1q antibody Lyophilized Standard 2 x XXX 100X Detector Antibody 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Complement 1q antibody Microplate 96 Wells (12 x 8 Well strips) Complement 1q antibody Microplate 96 Wells (12 x 8 Well strips) Complement 1q antibody Lyophilized Standard 2 x XXX Complement 1q antibody Lyophilized Standard 2 x XXX 100X Detector Antibody 1 x 120 uL 100X Detector Antibody 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
NCBI Gene Symbol
C1QA|C1QB|C1QC
Protein Name
Complement C1q subcomponent subunit A|Complement C1q subcomponent subunit B|Complement C1q subcomponent subunit C
Gene Name URL
C1QA|C1QB|C1QC

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