A2ML1 ELISA Kit (Human)

• Reproducibility :

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Alpha-2-Macroglobulin Like Protein 1 (a2ML1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Alpha-2-Macroglobulin Like Protein 1 (a2ML1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name :

  Alpha-2-macroglobulin like 1

• Gene Aliases :

  Alpha-2-macroglobulin-like protein 1; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 9; C3 and PZP-like, alpha-2-macroglobulin domain containing 9; CPAMD9; OMS; p170.

• Gene ID :

  144568

• Accession Number :

  NP_001269353

• Reactivity :

  Homo sapiens|Human

• Target :

  Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced) . Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity) . Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases.

• Type :

  ELISA Kit

• Applications :

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method :

  Colorimetric

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Macroglobulin Like Protein 1 (a2ML1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Macroglobulin Like Protein 1 (a2ML1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Macroglobulin Like Protein 1 (a2ML1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alpha-2-Macroglobulin Like Protein 1 (a2ML1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Serum, plasma, tissue homogenates and other biological fluids

• Detection Range :

  0.78-50ng/mL

• Recovery :

  Matrix Recovery Range (%) Average (%) _x005F serum (n=5) 83-103 98 _x005F EDTA plasma (n=5) 99-105 102 _x005F heparin plasma (n=5) 79-91 85 _x005F Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 83-103 98 serum (n=5) 83-103 98 EDTA plasma (n=5) 99-105 102 EDTA plasma (n=5) 99-105 102 heparin plasma (n=5) 79-91 85 heparin plasma (n=5) 79-91 85

• Sensitivity :

  < 0.34 ng/mL

• Reconstitution :

  2°C to 8°C|-20°C

• Components :

  Component Amount _x005F Anti-A2ML1 Microplate 96 Wells (12 x 8 Well Strips) _x005F A2ML1 Lyophilized Standard 2 x 50 ng _x005F 100X Biotinylated A2ML1 Detector Antibody 120 uL _x005F Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount Component Amount Anti-A2ML1 Microplate 96 Wells (12 x 8 Well Strips) Anti-A2ML1 Microplate 96 Wells (12 x 8 Well Strips) A2ML1 Lyophilized Standard 2 x 50 ng A2ML1 Lyophilized Standard 2 x 50 ng 100X Biotinylated A2ML1 Detector Antibody 120 uL 100X Biotinylated A2ML1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes :

  Formerly GWB-KBBMN3

• Specificity :

  This assay has high sensitivity and excellent specificity for detection of Alpha-2-Macroglobulin Like Protein 1 (a2ML1) ._x005F No significant cross-reactivity or interference between Alpha-2-Macroglobulin Like Protein 1 (a2ML1) and analogues was observed.

• Protein Length :

  1454

• NCBI Gene Symbol :

  A2ML1

• Protein Name :

  Alpha-2-macroglobulin-like protein 1

• Gene Name URL :

  A2ML1

• CAS Number :

  7732-18-5

• Nucleotide Accession Number :

  NM_001282424.2