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ITGA2 ELISA Kit (Human)

CAT: 0247-OKCD01197-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01197-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Integrin Alpha 1 (ITGa1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Integrin Alpha 1 (ITGa1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Integrin subunit alpha 1
Gene Aliases
CD49 antigen-like family member A; CD49a; integrin alpha-1; laminin and collagen receptor; very late activation protein 1; very late antigen-1; VLA1; VLA-1.
Gene ID
3672
Accession Number
XP_001075558
Reactivity
Homo sapiens|Human
Target
Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 1 (ITGa1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha 1 (ITGa1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 1 (ITGa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Integrin Alpha 1 (ITGa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
78-5,000pg/mL
Sensitivity
< 29 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-ITGA2 Microplate 96 Wells (12 x 8 Well Strips) ITGA2 Lyophilized Standard 2 x 5 ng 100X Biotinylated ITGA2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-ITGA2 Microplate 96 Wells (12 x 8 Well Strips) Anti-ITGA2 Microplate 96 Wells (12 x 8 Well Strips) ITGA2 Lyophilized Standard 2 x 5 ng ITGA2 Lyophilized Standard 2 x 5 ng 100X Biotinylated ITGA2 Detector Antibody 120 uL 100X Biotinylated ITGA2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBJT7
Specificity
This assay has high sensitivity and excellent specificity for detection of Integrin Alpha 1 (ITGa1) . No significant cross-reactivity or interference between Integrin Alpha 1 (ITGa1) and analogues was observed.
Protein Length
1181
NCBI Gene Symbol
ITGA1
Protein Name
Integrin alpha-1
Gene Name URL
ITGA1
Nucleotide Accession Number
XM_001075558.5

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