GREM1 ELISA Kit (Human)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Gremlin 1 (GREM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Gremlin 1 (GREM1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Gremlin 1, DAN family BMP antagonist

• Gene Aliases:

  C15DUPq; cell proliferation-inducing gene 2 protein; CKTSF1B1; colorectal adenoma and carcinoma 1; CRAC1; CRCS4; cysteine knot superfamily 1, BMP antagonist 1; DAN domain family member 2; DAND2; down-regulated in Mos-transformed cells protein; DRM; DUP15q; GREMLIN; gremlin 1, cysteine knot superfamily, homolog; gremlin 1-like protein; gremlin-1; hereditary mixed polyposis syndrome; HMPS; HMPS1; IHG-2; Increased in high glucose protein 2; increased in high glucose-2; MPSH; PIG2.

• Gene ID:

  26585

• Accession Number:

  NP_001178252

• Reactivity:

  Homo sapiens|Human

• Target:

  Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner (By similarity) . Antagonist of BMP2; inhibits BMP2-mediated differentiation of osteoblasts (in vitro) (PubMed:27036124) . Acts as inhibitor of monocyte chemotaxis. Can inhibit the growth or viability of normal cells but not transformed cells when is overexpressed (By similarity) .

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gremlin 1 (GREM1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gremlin 1 (GREM1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gremlin 1 (GREM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Gremlin 1 (GREM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates and other biological fluids

• Detection Range:

  6.25-400ng/mL

• Recovery:

  Matrix Recovery Range (%) Average (%) _x005F serum (n=5) 89-97 93 _x005F EDTA plasma (n=5) 80-89 85 _x005F heparin plasma (n=5) 78-97 83 _x005F Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 89-97 93 serum (n=5) 89-97 93 EDTA plasma (n=5) 80-89 85 EDTA plasma (n=5) 80-89 85 heparin plasma (n=5) 78-97 83 heparin plasma (n=5) 78-97 83

• Sensitivity:

  < 0.262 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount _x005F Anti-GREM1 Microplate 96 Wells (12 x 8 Well Strips) _x005F GREM1 Lyophilized Standard 2 x 40 ng _x005F 100X Biotinylated GREM1 Detector Antibody 120 uL _x005F Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount Component Amount Anti-GREM1 Microplate 96 Wells (12 x 8 Well Strips) Anti-GREM1 Microplate 96 Wells (12 x 8 Well Strips) GREM1 Lyophilized Standard 2 x 40 ng GREM1 Lyophilized Standard 2 x 40 ng 100X Biotinylated GREM1 Detector Antibody 120 uL 100X Biotinylated GREM1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes:

  Formerly GWB-KBBWE3

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Gremlin 1 (GREM1) ._x005F No significant cross-reactivity or interference between Gremlin 1 (GREM1) and analogues was observed.

• Protein Length:

  184

• NCBI Gene Symbol:

  GREM1

• Protein Name:

  Gremlin-1

• Gene Name URL:

  GREM1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001191323.1