CD142 ELISA Kit (Rabbit)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Tissue Factor (TF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Tissue Factor (TF) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Coagulation factor III, tissue factor

• Gene Aliases:

  Brain tissue factor; Coagulation factor III; coagulation factor III (thromboplastin, tissue factor) ; TF; tissue factor.

• Gene ID:

  100009127

• Accession Number:

  NP_001075760

• Reactivity:

  Oryctolagus cuniculus|Rabbit

• Target:

  Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tissue Factor (TF) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tissue Factor (TF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tissue Factor (TF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tissue Factor (TF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

• Detection Range:

  6.25-400pg/mL

• Recovery:

  Matrix Recovery Range (%) Average (%) serum (n=5) 89-96 92 EDTA plasma (n=5) 80-90 87 heparin plasma (n=5) 82-89 85 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 89-96 92 serum (n=5) 89-96 92 EDTA plasma (n=5) 80-90 87 EDTA plasma (n=5) 80-90 87 heparin plasma (n=5) 82-89 85 heparin plasma (n=5) 82-89 85

• Sensitivity:

  < 21.6 pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-CD142 Microplate 96 Wells (12 x 8 Well Strips) CD142 Lyophilized Standard 2 x 4 ng 100X Biotinylated CD142 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-CD142 Microplate 96 Wells (12 x 8 Well Strips) Anti-CD142 Microplate 96 Wells (12 x 8 Well Strips) CD142 Lyophilized Standard 2 x 4 ng CD142 Lyophilized Standard 2 x 4 ng 100X Biotinylated CD142 Detector Antibody 120 uL 100X Biotinylated CD142 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes:

  Formerly GWB-KBBPA2

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Tissue Factor (TF) . No significant cross-reactivity or interference between Tissue Factor (TF) and analogues was observed.

• Protein Length:

  292

• NCBI Gene Symbol:

  F3

• Protein Name:

  Tissue factor

• Gene Name URL:

  F3

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001082291.1