CD14 ELISA Kit (Mouse)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 0.98 3.11 6.23 1.03 3.47 6.72 SD 0.038 0.162 0.355 0.081 0.249 0.423 CV% 3.9 5.2 5.7 7.9 7.2 6.3 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 0.98 3.11 6.23 1.03 3.47 6.72 Mean (pg/mL) 0.98 3.11 6.23 1.03 3.47 6.72 SD 0.038 0.162 0.355 0.081 0.249 0.423 SD 0.038 0.162 0.355 0.081 0.249 0.423 CV% 3.9 5.2 5.7 7.9 7.2 6.3 CV% 3.9 5.2 5.7 7.9 7.2 6.3

• Gene Name:

  CD14 molecule

• Gene Aliases:

  CD 14, CD14 antigen, CD14 molecule, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

• Gene ID:

  12475

• Accession Number:

  NP_033971.1

• Reactivity:

  Mouse

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: A18-P345

• Target:

  CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS) . CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP) . Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's mouse CD14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD14 has been precoated onto 96-well plates. Standards (NS0, A18-P345) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CD14 amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernatants, serum and plasma (heparin, EDTA)

• Detection Range:

  0.78 ng/ml - 50 ng/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant mouse CD14

• NCBI Gene Symbol:

  CD14

• Protein Name:

  Monocyte differentiation antigen CD14

• Gene Name URL:

  CD14

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_009841.4