CD14 ELISA Kit (Mouse)

CAT: 0247-OKBB00420-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB00420-96WSize:96 Well With Removable Strips
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 0.98 3.11 6.23 1.03 3.47 6.72 SD 0.038 0.162 0.355 0.081 0.249 0.423 CV% 3.9 5.2 5.7 7.9 7.2 6.3 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 0.98 3.11 6.23 1.03 3.47 6.72 Mean (pg/mL) 0.98 3.11 6.23 1.03 3.47 6.72 SD 0.038 0.162 0.355 0.081 0.249 0.423 SD 0.038 0.162 0.355 0.081 0.249 0.423 CV% 3.9 5.2 5.7 7.9 7.2 6.3 CV% 3.9 5.2 5.7 7.9 7.2 6.3
CAS Number
7732-18-5
Gene Name
CD14 molecule
Gene Aliases
CD 14, CD14 antigen, CD14 molecule, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
Gene ID
12475
Accession Number
NP_033971.1
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: A18-P345
Target
CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS) . CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP) . Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse CD14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD14 has been precoated onto 96-well plates. Standards (NS0, A18-P345) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CD14 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
0.78 ng/ml - 50 ng/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse CD14
NCBI Gene Symbol
CD14
Protein Name
Monocyte differentiation antigen CD14
Gene Name URL
CD14
Nucleotide Accession Number
NM_009841.4

Related Products

CatalogName
OKEH03188-96WCD14 ELISA Kit (Rat)
OKCD06667-96WCD14 ELISA Kit (Human)
OKCD01173-96WCD14 ELISA Kit (Pig)
OKEH02674-96WCD14 ELISA Kit (Human)
OKCA01002-96WCD14 ELISA Kit (Rat)
OKBB00782-96WCD14 ELISA Kit (Human)
OKCD06669-96WCD14 ELISA Kit (Rat)
OKEH07451-96WCD14 ELISA Kit (Bovine)
OKCD06667CD14 ELISA Kit (Human) (OKCD06667)
OKBB00782CD14 ELISA Kit (Human) (OKBB00782)

Alternative Products

CatalogName
YP-W20036-01Mouse CD14 (CD14) ELISA Kit
KT-1001Mouse CD14 ELISA
EA100441Mouse CD14 ELISA Kit
MBS729343-01Mouse CD14  ELISA Kit
LF-EK50879Mouse CD14 ELISA Kit
DY982Mouse CD14 DuoSet ELISA
F0836Monocyte differentiation antigen CD14 (CD14) Mouse ELISA Kit
EK00333EMouse Cd14/Monocyte differentiation antigen CD14 ELISA Kit
RK07159Mouse Monocyte differentiation antigen CD14 (CD14) ELISA Kit
JOT-EK2267MoMouse Monocyte differentiation antigen CD14,CD14 ELISA KIT

Popular Products