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EZH1 ELISA Kit (Human)

CAT: 0247-OKCD01063-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01063-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Enhancer Of Zeste Homolog 1 (EZH1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Enhancer Of Zeste Homolog 1 (EZH1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Enhancer of zeste 1 polycomb repressive complex 2 subunit
Gene Aliases
Enhancer of zeste homolog 1; ENX-2; histone-lysine N-methyltransferase EZH1; KMT6B.
Gene ID
2145
Accession Number
NP_001308008
Reactivity
Homo sapiens|Human
Target
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enhancer Of Zeste Homolog 1 (EZH1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Enhancer Of Zeste Homolog 1 (EZH1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Enhancer Of Zeste Homolog 1 (EZH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Enhancer Of Zeste Homolog 1 (EZH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
< 0.113 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-EZH1 Microplate 96 Wells (12 x 8 Well Strips) EZH1 Lyophilized Standard 2 x 20 ng 100X Biotinylated EZH1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-EZH1 Microplate 96 Wells (12 x 8 Well Strips) Anti-EZH1 Microplate 96 Wells (12 x 8 Well Strips) EZH1 Lyophilized Standard 2 x 20 ng EZH1 Lyophilized Standard 2 x 20 ng 100X Biotinylated EZH1 Detector Antibody 120 uL 100X Biotinylated EZH1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBYS6
Specificity
This assay has high sensitivity and excellent specificity for detection of Enhancer Of Zeste Homolog 1 (EZH1) . No significant cross-reactivity or interference between Enhancer Of Zeste Homolog 1 (EZH1) and analogues was observed.
Protein Length
747
NCBI Gene Symbol
EZH1
Protein Name
Histone-lysine N-methyltransferase EZH1
Gene Name URL
EZH1
Nucleotide Accession Number
NM_001321079.1

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