PPM1A ELISA Kit (Human)

CAT: 0247-OKCD01029-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD01029-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Protein phosphatase, Mg2+/Mn2+ dependent 1A
Gene Aliases
PP2CA; PP2Calpha; PP2C-ALPHA; protein phosphatase 1A; Protein phosphatase 2C isoform alpha; Protein phosphatase IA.
Gene ID
5494
Accession Number
NP_066283
Reactivity
Homo sapiens|Human
Target
Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
< 0.123 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-PPM1A Microplate 96 Wells (12 x 8 Well Strips) PPM1A Lyophilized Standard 2 x 20 ng 100X Biotinylated PPM1A Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-PPM1A Microplate 96 Wells (12 x 8 Well Strips) Anti-PPM1A Microplate 96 Wells (12 x 8 Well Strips) PPM1A Lyophilized Standard 2 x 20 ng PPM1A Lyophilized Standard 2 x 20 ng 100X Biotinylated PPM1A Detector Antibody 120 uL 100X Biotinylated PPM1A Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBLL1
Specificity
This assay has high sensitivity and excellent specificity for detection of Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) . No significant cross-reactivity or interference between Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) and analogues was observed.
Protein Length
382
NCBI Gene Symbol
PPM1A
Protein Name
Protein phosphatase 1A
Gene Name URL
PPM1A
Nucleotide Accession Number
NM_021003.4

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