SPINT2 ELISA Kit (Human)

CAT: 0247-OKCD01024-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD01024-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Serine Peptidase Inhibitor Kunitz Type 2 (SPINT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Serine Peptidase Inhibitor Kunitz Type 2 (SPINT2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Serine peptidase inhibitor, Kunitz type 2
Gene Aliases
DIAR3; HAI2; HAI-2; hepatocyte growth factor activator inhibitor type 2; Kop; kunitz-type protease inhibitor 2; PB; Placental bikunin; serine protease inhibitor, Kunitz type, 2; testicular tissue protein Li 183.
Gene ID
10653
Accession Number
NP_001159575
Reactivity
Homo sapiens|Human
Target
Inhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Peptidase Inhibitor Kunitz Type 2 (SPINT2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Peptidase Inhibitor Kunitz Type 2 (SPINT2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Peptidase Inhibitor Kunitz Type 2 (SPINT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Serine Peptidase Inhibitor Kunitz Type 2 (SPINT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids.
Detection Range
0.312-20ng/mL
Recovery
Matrix Recovery Range (%) Average (%) serum (n=5) 96-103 99 EDTA plasma (n=5) 84-94 87 heparin plasma (n=5) 87-104 97 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 96-103 99 serum (n=5) 96-103 99 EDTA plasma (n=5) 84-94 87 EDTA plasma (n=5) 84-94 87 heparin plasma (n=5) 87-104 97 heparin plasma (n=5) 87-104 97
Sensitivity
< 0.103 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-SPINT2 Microplate 96 Wells (12 x 8 Well Strips) SPINT2 Lyophilized Standard 2 x 20 ng 100X Biotinylated SPINT2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-SPINT2 Microplate 96 Wells (12 x 8 Well Strips) Anti-SPINT2 Microplate 96 Wells (12 x 8 Well Strips) SPINT2 Lyophilized Standard 2 x 20 ng SPINT2 Lyophilized Standard 2 x 20 ng 100X Biotinylated SPINT2 Detector Antibody 120 uL 100X Biotinylated SPINT2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBVH3
Specificity
This assay has high sensitivity and excellent specificity for detection of Serine Peptidase Inhibitor Kunitz Type 2 (SPINT2) . No significant cross-reactivity or interference between Serine Peptidase Inhibitor Kunitz Type 2 (SPINT2) and analogues was observed.
Protein Length
252
NCBI Gene Symbol
SPINT2
Protein Name
Kunitz-type protease inhibitor 2
Gene Name URL
SPINT2
Nucleotide Accession Number
NM_001166103.1

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