THBS2 ELISA Kit (Mouse)

CAT: 0247-OKCD00921-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD00921-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Thrombospondin 2 (THBS2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Thrombospondin 2 (THBS2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Thrombospondin 2
Gene Aliases
Thbs-; Thbs-2; thrombospondin-2; TS; TSP2.
Gene ID
21826
Accession Number
NP_001162609
Reactivity
Mouse|Mus musculus
Target
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombospondin 2 (THBS2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thrombospondin 2 (THBS2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thrombospondin 2 (THBS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thrombospondin 2 (THBS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Plasma, tissue homogenates and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Matrix Recovery Range (%) Average (%) sodium citrate plasma (n=5) 84-102 88 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) sodium citrate plasma (n=5) 84-102 88 sodium citrate plasma (n=5) 84-102 88
Sensitivity
< 0.137 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Thbs2 Microplate 96 Wells (12 x 8 Well Strips) Thbs2 Lyophilized Standard 2 x 20 ng 100X Biotinylated Thbs2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Thbs2 Microplate 96 Wells (12 x 8 Well Strips) Anti-Thbs2 Microplate 96 Wells (12 x 8 Well Strips) Thbs2 Lyophilized Standard 2 x 20 ng Thbs2 Lyophilized Standard 2 x 20 ng 100X Biotinylated Thbs2 Detector Antibody 120 uL 100X Biotinylated Thbs2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBML2
Specificity
This assay has high sensitivity and excellent specificity for detection of Thrombospondin 2 (THBS2) . No significant cross-reactivity or interference between Thrombospondin 2 (THBS2) and analogues was observed.
Protein Length
1172
NCBI Gene Symbol
Thbs2
Protein Name
Thrombospondin-2
Gene Name URL
Thbs2
Nucleotide Accession Number
NM_001169138.1

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