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SNX13 ELISA Kit (Human)

CAT: 0247-OKCD00863-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD00863-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Sorting Nexin 13 (SNX13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Sorting Nexin 13 (SNX13) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Sorting nexin 13
Gene Aliases
Rgs domain- and phox domain-containing protein; RGS-PX1; sorting nexin-13.
Gene ID
23161
Accession Number
NP_055947
Reactivity
Homo sapiens|Human
Target
May be involved in several stages of intracellular trafficking. May play a role in endosome homeostasis (By similarity) . Acts as a GAP for Galphas.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sorting Nexin 13 (SNX13) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sorting Nexin 13 (SNX13) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sorting Nexin 13 (SNX13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sorting Nexin 13 (SNX13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
< 0.128 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-SNX13 Microplate 96 Wells (12 x 8 Well Strips) SNX13 Lyophilized Standard 2 x 20 ng 100X Biotinylated SNX13 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-SNX13 Microplate 96 Wells (12 x 8 Well Strips) Anti-SNX13 Microplate 96 Wells (12 x 8 Well Strips) SNX13 Lyophilized Standard 2 x 20 ng SNX13 Lyophilized Standard 2 x 20 ng 100X Biotinylated SNX13 Detector Antibody 120 uL 100X Biotinylated SNX13 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBMN7
Specificity
This assay has high sensitivity and excellent specificity for detection of Sorting Nexin 13 (SNX13) . No significant cross-reactivity or interference between Sorting Nexin 13 (SNX13) and analogues was observed.
Protein Length
968
NCBI Gene Symbol
SNX13
Protein Name
Sorting nexin-13
Gene Name URL
SNX13
Nucleotide Accession Number
NM_015132.4

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