BMPR2 ELISA Kit (Human)

CAT: 0247-OKCD00783-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD00783-96WSize:96 Well
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 2 (BMPR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 2 (BMPR2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Bone morphogenetic protein receptor type 2
Gene Aliases
BMP type II receptor; BMP type-2 receptor; BMPR3; BMPR-II; BMR2; bone morphogenetic protein receptor type II; bone morphogenetic protein receptor type-2; bone morphogenetic protein receptor, type II (serine/threonine kinase) ; BRK-3; POVD1; PPH1; T-ALK; type II activin receptor-like kinase; type II receptor for bone morphogenetic protein-4.
Gene ID
659
Accession Number
NP_001195
Reactivity
Homo sapiens|Human
Target
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Binds to BMP7, BMP2 and, less efficiently, BMP4. Binding is weak but enhanced by the presence of type I receptors for BMPs. Mediates induction of adipogenesis by GDF6.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein Receptor 2 (BMPR2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein Receptor 2 (BMPR2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein Receptor 2 (BMPR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bone Morphogenetic Protein Receptor 2 (BMPR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery Range (%) Average (%) 94-102 98 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) 94-102 98 94-102 98
Sensitivity
< 0.124 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-BMPR2 Microplate 96 Wells (12 x 8 Well Strips) BMPR2 Lyophilized Standard 2 x 20 ng 100X Biotinylated BMPR2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-BMPR2 Microplate 96 Wells (12 x 8 Well Strips) Anti-BMPR2 Microplate 96 Wells (12 x 8 Well Strips) BMPR2 Lyophilized Standard 2 x 20 ng BMPR2 Lyophilized Standard 2 x 20 ng 100X Biotinylated BMPR2 Detector Antibody 120 uL 100X Biotinylated BMPR2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBUQ1
Specificity
This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein Receptor 2 (BMPR2) . No significant cross-reactivity or interference between Bone Morphogenetic Protein Receptor 2 (BMPR2) and analogues was observed.
Protein Length
1038
NCBI Gene Symbol
BMPR2
Protein Name
Bone morphogenetic protein receptor type-2
Gene Name URL
BMPR2
Nucleotide Accession Number
NM_001204.6

Related Products

CatalogName
OKEH05926-96WBMPR2 ELISA Kit (Mouse)
OKWB00350BMPR2 ELISA Kit (Rat) (OKWB00350)
OKEI00700BMPR2 ELISA Kit (Rat) (OKEI00700)
OKCA02346BMPR2 ELISA Kit (Mouse) (OKCA02346)
OKEH05926BMPR2 ELISA Kit (Mouse) (OKEH05926)
OKCA02346-96WOKCA02346-96W - BMPR2 ELISA Kit (Mouse)
OAAL00028-100UGBMPR2 Antibody
OAAJ06366-100ULBMPR2 Antibody
OAAJ07914-100ULBMPR2 Antibody
OCOA14702-20UGBMPR2 Protein Lysate

Alternative Products

CatalogName
EF008128Human BMPR2 ELISA KIT
SYP-H0177-01Human BMPR2(Bone Morphogenetic Protein Receptor Ⅱ) ELISA Kit
FY-EH1939Human Bmpr2 (Bone Morphogenetic Protein Receptor II) ELISA Kit
MBS8804152-01Human BMPR2 (Bone Morphogenetic Protein Receptor 2) ELISA Kit
MBS2024927-01Human Bone Morphogenetic Protein Receptor 2 (BMPR2) ELISA Kit
KBH1563GENLISA™ Human Bone Morphogenetic Protein Receptor 2 (BMPR2) ELISA
FY-EH1325SEasypro Human Bone Morphogenetic Protein Receptor II (BMPR2) ELISA Kit
MBS2571757-01Uncoated Human BMPR2 (Bone Morphogenetic Protein Receptor II ) ELISA Kit
E0290HuHuman BMPR2/Bone morphogenetic protein receptor type-2 ELISA Kit
365781-01Bone Morphogenetic Protein Receptor 2 (BMPR2) BioAssayTM ELISA Kit (Human)

Popular Products