Products for Research Use Only
RegisterLogin

CKMT2 ELISA Kit (Human)

CAT: 0247-OKCD00684-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD00684-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Creatine kinase, mitochondrial 2
Gene Aliases
Basic-type mitochondrial creatine kinase; creatine kinase S-type, mitochondrial; creatine kinase, mitochondrial 2 (sarcomeric) ; mib-CK; sarcomeric mitochondrial creatine kinase; S-MtCK; SMTCK.
Gene ID
1160
Accession Number
NP_001093205
Reactivity
Homo sapiens|Human
Target
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) . Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
1.56-100ng/mL
Recovery
Matrix Recovery Range (%) Average (%) 79-91 86 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) 79-91 86 79-91 86
Sensitivity
< 0.63 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-CKMT2 Microplate 96 Wells (12 x 8 Well Strips) CKMT2 Lyophilized Standard 2 x 100 ng 100X Biotinylated CKMT2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-CKMT2 Microplate 96 Wells (12 x 8 Well Strips) Anti-CKMT2 Microplate 96 Wells (12 x 8 Well Strips) CKMT2 Lyophilized Standard 2 x 100 ng CKMT2 Lyophilized Standard 2 x 100 ng 100X Biotinylated CKMT2 Detector Antibody 120 uL 100X Biotinylated CKMT2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBVO1
Specificity
This assay has high sensitivity and excellent specificity for detection of Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2) . No significant cross-reactivity or interference between Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2) and analogues was observed.
Protein Length
419
NCBI Gene Symbol
CKMT2
Protein Name
Creatine kinase S-type, mitochondrial
Gene Name URL
CKMT2
Nucleotide Accession Number
NM_001099735.1

Related Products

CatalogName
OKAG01201CKMT2 Colorimetric Cell-Based ELISA Kit (OKAG01201)
OACD02553-1MLCKMT2 Antibody
OACD02553-100ULCKMT2 Antibody
OAGA02972-100ULCKMT2 Antibody
OACA07854-100ULCKMT2 Antibody
OAAJ03993-100ULCKMT2 Antibody
OACA07854-50ULCKMT2 Antibody
OAAB21050-100ULCKMT2 Antibody
OAGA03856-100ULCKMT2 Antibody
OCOA00619-20UGCKMT2 Protein Lysate

Alternative Products

CatalogName
E010186Human CKMT2 ELISA Kit
MBS2019508-01Human Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2) ELISA Kit
E5124Hu-01Human Creatine kinase S-type, mitochondrial, CKMT2 ELISA KIT
EK244424Human CKMT2 (Creatine Kinase, Mitochondrial 2, Sarcomeric) ELISA Kit
KBH5124GENLISA™ Human Creatine Kinase Mitochondrial 2 Sarcomeric (CKMT2) ELISA
MBS7246557-01Human Creatine kinase S type, mitochondrial (CKMT2) ELISA Kit
C6141Creatine Kinase S-type mitochondrial (CKMT2) Human ELISA Kit
JOT-EK5124Hu-01Human Creatine kinase S-type, mitochondrial,CKMT2 ELISA KIT
MBS2905412-01Human CKMT2 / Creatine kinase S-type, mitochondrial ELISA Kit
JL52480-01Human Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2) ELISA Kit

Popular Products