TWF1 ELISA Kit (Human)

CAT: 0247-OKCD00620-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD00620-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Twinfilin 1 (TWF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Twinfilin 1 (TWF1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Twinfilin actin binding protein 1
Gene Aliases
A6; A6 protein tyrosine kinase; protein A6; protein tyrosine kinase 9; PTK9; PTK9 protein tyrosine kinase 9; twinfilin, actin-binding protein, homolog 1; twinfilin-1.
Gene ID
5756
Accession Number
NP_001229326
Reactivity
Homo sapiens|Human
Target
Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Twinfilin 1 (TWF1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Twinfilin 1 (TWF1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Twinfilin 1 (TWF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Twinfilin 1 (TWF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.054 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-TWF1 Microplate 96 Wells (12 x 8 Well Strips) TWF1 Lyophilized Standard 2 x 10 ng 100X Biotinylated TWF1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-TWF1 Microplate 96 Wells (12 x 8 Well Strips) Anti-TWF1 Microplate 96 Wells (12 x 8 Well Strips) TWF1 Lyophilized Standard 2 x 10 ng TWF1 Lyophilized Standard 2 x 10 ng 100X Biotinylated TWF1 Detector Antibody 120 uL 100X Biotinylated TWF1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBFX5
Specificity
This assay has high sensitivity and excellent specificity for detection of Twinfilin 1 (TWF1) . No significant cross-reactivity or interference between Twinfilin 1 (TWF1) and analogues was observed.
Protein Length
350
NCBI Gene Symbol
TWF1
Protein Name
Twinfilin-1
Gene Name URL
TWF1
Nucleotide Accession Number
NM_001242397.1

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