HSD17B14 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

7732-18-5

Hydroxysteroid 17-beta dehydrogenase 14

17-beta-HSD 14;17-beta-hydroxysteroid dehydrogenase 14;17-beta-hydroxysteroid dehydrogenase DHRS10; dehydrogenase/reductase (SDR family) member 10; Dehydrogenase/reductase SDR family member 10; DHRS10; retinal short-chain dehydrogenase/reductase 3; retinal short-chain dehydrogenase/reductase retSDR3; retSDR3; SDR47C1; short chain dehydrogenase/reductase family 47C member 1; testicular tissue protein Li 52.

51171

NP_057330

Homo sapiens|Human

Has NAD-dependent 17-beta-hydroxysteroid dehydrogenase activity. Converts oestradiol to oestrone. The physiological substrate is not known. Acts on oestradiol and 5-androstene-3-beta,17-beta-diol (in vitro) .

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Tissue homogenates, cell lysates and other biological fluids

0.156-10ng/mL

< 0.055 ng/mL

2°C to 8°C|-20°C

Component Amount Anti-HSD17B14 Microplate 96 Wells (12 x 8 Well Strips) HSD17B14 Lyophilized Standard 2 x 10 ng 100X Biotinylated HSD17B14 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-HSD17B14 Microplate 96 Wells (12 x 8 Well Strips) Anti-HSD17B14 Microplate 96 Wells (12 x 8 Well Strips) HSD17B14 Lyophilized Standard 2 x 10 ng HSD17B14 Lyophilized Standard 2 x 10 ng 100X Biotinylated HSD17B14 Detector Antibody 120 uL 100X Biotinylated HSD17B14 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

Formerly GWB-KBBTT7

This assay has high sensitivity and excellent specificity for detection of 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) . No significant cross-reactivity or interference between 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) and analogues was observed.

270

HSD17B14

17-beta-hydroxysteroid dehydrogenase 14

HSD17B14