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NR5A2 ELISA Kit (Rat)

CAT: 0247-OKCD00412-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD00412-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Liver Receptor Homolog 1 (LRH1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Liver Receptor Homolog 1 (LRH1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Nuclear receptor subfamily 5, group A, member 2
Gene Aliases
Fetoprotein transcription factor; Ftf; FTZ-F1 beta; FTZ-F1 beta2 protein; liver receptor homolog 1; Lrh-1; nuclear receptor subfamily 5 group A member 2.
Gene ID
60349
Accession Number
NP_068510
Reactivity
Rat|Rattus norvegicus
Target
Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Liver Receptor Homolog 1 (LRH1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Liver Receptor Homolog 1 (LRH1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Liver Receptor Homolog 1 (LRH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Liver Receptor Homolog 1 (LRH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.063 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Nr5a2 Microplate 96 Wells (12 x 8 Well Strips) Nr5a2 Lyophilized Standard 2 x 10 ng 100X Biotinylated Nr5a2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Nr5a2 Microplate 96 Wells (12 x 8 Well Strips) Anti-Nr5a2 Microplate 96 Wells (12 x 8 Well Strips) Nr5a2 Lyophilized Standard 2 x 10 ng Nr5a2 Lyophilized Standard 2 x 10 ng 100X Biotinylated Nr5a2 Detector Antibody 120 uL 100X Biotinylated Nr5a2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBQV5
Specificity
This assay has high sensitivity and excellent specificity for detection of Liver Receptor Homolog 1 (LRH1) . No significant cross-reactivity or interference between Liver Receptor Homolog 1 (LRH1) and analogues was observed.
Protein Length
560
NCBI Gene Symbol
Nr5a2
Protein Name
Nuclear receptor subfamily 5 group A member 2
Gene Name URL
Nr5a2
Nucleotide Accession Number
NM_021742.1

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