ERBB4 ELISA Kit (Human)

CAT: 0247-OKCD00310-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD00310-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Erb-b2 Receptor Tyrosine Kinase 4 (ErbB4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Erb-b2 Receptor Tyrosine Kinase 4 (ErbB4) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Erb-b2 receptor tyrosine kinase 4
Gene Aliases
ALS19; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; HER4; human epidermal growth factor receptor 4; p180erbB4; proto-oncogene-like protein c-ErbB-4; receptor tyrosine-protein kinase erbB-4; tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4.
Gene ID
2066
Accession Number
NP_001036064
Reactivity
Homo sapiens|Human
Target
Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Erb-b2 Receptor Tyrosine Kinase 4 (ErbB4) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Erb-b2 Receptor Tyrosine Kinase 4 (ErbB4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Erb-b2 Receptor Tyrosine Kinase 4 (ErbB4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Erb-b2 Receptor Tyrosine Kinase 4 (ErbB4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery Range (%) Average (%) serum (n=5) 93-102 98 EDTA plasma (n=5) 80-90 82 heparin plasma (n=5) 82-101 89 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 93-102 98 serum (n=5) 93-102 98 EDTA plasma (n=5) 80-90 82 EDTA plasma (n=5) 80-90 82 heparin plasma (n=5) 82-101 89 heparin plasma (n=5) 82-101 89
Sensitivity
< 0.062 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-ERBB4 Microplate 96 Wells (12 x 8 Well Strips) ERBB4 Lyophilized Standard 2 x 10 ng 100X Biotinylated ERBB4 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-ERBB4 Microplate 96 Wells (12 x 8 Well Strips) Anti-ERBB4 Microplate 96 Wells (12 x 8 Well Strips) ERBB4 Lyophilized Standard 2 x 10 ng ERBB4 Lyophilized Standard 2 x 10 ng 100X Biotinylated ERBB4 Detector Antibody 120 uL 100X Biotinylated ERBB4 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBZA4
Specificity
This assay has high sensitivity and excellent specificity for detection of V-Erb A Erythroblastic Leukemia Viral Oncogene Homolog 4 (ErbB4) . No significant cross-reactivity or interference between V-Erb A Erythroblastic Leukemia Viral Oncogene Homolog 4 (ErbB4) and analogues was observed.
Protein Length
1308
NCBI Gene Symbol
ERBB4
Protein Name
Receptor tyrosine-protein kinase erbB-4
Gene Name URL
ERBB4
Nucleotide Accession Number
NM_001042599.1

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