SCUBE1 ELISA Kit (Mouse)

• Reproducibility:

  Mean Inter-Assay: CV

• Gene Aliases:

  7330410C13Rik, A630023E24Rik

• Gene ID:

  64706

• Accession Number:

  NP_001258401

• Reactivity:

  Mouse

• Target:

  Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.

• Type:

  ELISA Kit

• Assay Principle:

  Aviva Systems Biology Scube1 ELISA Kit (Mouse) (OKCA02453) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Scube1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Scube1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Scube1 captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates

• Detection Range:

  0.156 - 10 ng/mL

• Recovery:

  Sample Type Mean Recovery (%) Range (%) Serum (n=5) 92 87-97 EDTA Plasma (n=4) 107 99-111 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 92 87-97 Serum (n=5) 92 87-97 EDTA Plasma (n=4) 107 99-111 EDTA Plasma (n=4) 107 99-111

• Sensitivity:

  0.039 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• NCBI Gene Symbol:

  SCUBE1

• Protein Name:

  Signal peptide, CUB and EGF-like domain-containing protein 1

• Gene Name URL:

  SCUBE1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001271472