ITGAM ELISA Kit (Mouse)

• Reproducibility:

  Mean Inter-Assay: CV

• Gene Aliases:

  CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A, SLEB6, antigen CD11b (p170), integrin alpha M, macrophage antigen alpha polypeptide, neutrophil adherence receptor alpha-M subunit

• Reactivity:

  Mouse

• Target:

  Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Alpha-M/beta-2 play a critical role in mast cell development and in immune complex-mediated glomerulonephritis. Mice expressing a null mutation of the alpha-M subunit gene demonstrate increase in neutrophil accumulation, in response to a impaired degranulation and phagocytosis, events that apparently accelerate apoptosis in neutrophils. These mice develop obesity.

• Type:

  ELISA Kit

• Assay Principle:

  Aviva Systems Biology Itgam ELISA Kit (Mouse) (OKCA02400) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Itgam has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Itgam is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Itgam captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Cell Culture Supernates, Tissue Homogenates

• Detection Range:

  15.6 - 1,000 pg/mL

• Recovery:

  Sample Type Mean Recovery (%) Range (%) Serum (n=5) 95 90-100 EDTA Plasma (n=4) 88 84-92 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 95 90-100 Serum (n=5) 95 90-100 EDTA Plasma (n=4) 88 84-92 EDTA Plasma (n=4) 88 84-92

• Sensitivity:

  3.9 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• NCBI Gene Symbol:

  ITGAM

• Protein Name:

  Integrin alpha-M

• Gene Name URL:

  ITGAM

• CAS Number:

  7732-18-5