ITGAM ELISA Kit (Mouse)

CAT: 0247-OKCA02400-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCA02400-96WSize:96 Well
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Reproducibility
Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Aliases
CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A, SLEB6, antigen CD11b (p170), integrin alpha M, macrophage antigen alpha polypeptide, neutrophil adherence receptor alpha-M subunit
Reactivity
Mouse
Target
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Alpha-M/beta-2 play a critical role in mast cell development and in immune complex-mediated glomerulonephritis. Mice expressing a null mutation of the alpha-M subunit gene demonstrate increase in neutrophil accumulation, in response to a impaired degranulation and phagocytosis, events that apparently accelerate apoptosis in neutrophils. These mice develop obesity.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Itgam ELISA Kit (Mouse) (OKCA02400) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Itgam has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Itgam is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Itgam captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Culture Supernates, Tissue Homogenates
Detection Range
15.6 - 1,000 pg/mL
Recovery
Sample Type Mean Recovery (%) Range (%) Serum (n=5) 95 90-100 EDTA Plasma (n=4) 88 84-92 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 95 90-100 Serum (n=5) 95 90-100 EDTA Plasma (n=4) 88 84-92 EDTA Plasma (n=4) 88 84-92
Sensitivity
3.9 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
ITGAM
Protein Name
Integrin alpha-M
Gene Name URL
ITGAM

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