ITGAM Antibody

• Gene Name:

  Integrin alpha M

• Gene Aliases:

  CD11 antigen-like family member B; Cd11b; CD11B (p170) ; CD11b/CD18; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement receptor type 3; CR; CR3; CR-3 alpha chain; CR3A; F730045J24Rik; integrin alpha-M; leukocyte adhesion receptor MO1; Ly-4; Ly-40; Mac-; Mac-1; MAC1; Mac-1 alpha; Mac-1a; macrophage antigen alpha.

• Gene ID:

  16409

• Accession Number:

  NP_000623.2NP_001139280.1

• Reactivity:

  Human|Mouse|Non-human Primate|Rabbit

• Immunogen:

  B10 mouse spleen cells enriched for T cells

• Target:

  CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18) ; expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

• Clonality:

  Monoclonal

• Clone:

  M1/70

• Type:

  Monoclonal Antibody

• Applications:

  Flow cytometry|Functional assay|Immunohistochemistry-Frozen|Immunoprecipitation

• Purification:

  Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Phosphate buffered saline (PBS), pH 7.4

• Reconstitution:

  2°C to 8°C

• Fragment:

  IgG2b

• Specificity:

  The rat monoclonal antibody M1/70 detects CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.

• NCBI Gene Symbol:

  Itgam

• Host or Source:

  Rat

• Protein Name:

  Integrin alpha-M

• Gene Name URL:

  Itgam

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_000632.3NM_001145808.1