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GPC3 ELISA Kit (Mouse)

CAT: 0247-OKCA02389-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA02389-96WSize:96 Well
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Reproducibility
Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Aliases
OCI, OCI-5
Gene ID
14734
Accession Number
NP_057906
Reactivity
Mouse
Target
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Gpc3 ELISA Kit (Mouse) (OKCA02389) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gpc3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gpc3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Gpc3 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Culture Supernates
Detection Range
3.12 - 200 ng/mL
Recovery
Sample Type Mean Recovery (%) Range (%) Serum (n=5) 99 91-103 EDTA Plasma (n=4) 95 90-99 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 99 91-103 Serum (n=5) 99 91-103 EDTA Plasma (n=4) 95 90-99 EDTA Plasma (n=4) 95 90-99
Sensitivity
0.78 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
GPC3
Protein Name
Glypican-3
Gene Name URL
GPC3
Nucleotide Accession Number
NM_016697

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