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ADAM17 ELISA Kit (Mouse)

CAT: 0247-OKCA02169-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA02169-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Variation
CAS Number
7732-18-5
Gene Aliases
Tac, Tace, CD156b
Gene ID
11491
Accession Number
NP_001264195
Reactivity
Mouse
Target
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Plays a role in the proteolytic processing of ACE2.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Adam17 ELISA Kit (Mouse) (OKCA02169) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAM17 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAM17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ADAM17 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma And Tissue Homogenates
Detection Range
15.6 pg/mL-1,000 pg/mL
Recovery
Sample Type Mean (%) Range (%) Serum (n=5) 95 90-100 EDTA plasma (n=4) 89 83-95 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 95 90-100 Serum (n=5) 95 90-100 EDTA plasma (n=4) 89 83-95 EDTA plasma (n=4) 89 83-95
Sensitivity
3.9 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
ADAM17
Protein Name
Disintegrin and metalloproteinase domain-containing protein 17
Gene Name URL
ADAM17
Nucleotide Accession Number
NM_001277266

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