CLEC7A ELISA Kit (Human)

CAT: 0247-OKCA02090-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCA02090-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Variation
CAS Number
7732-18-5
Gene Aliases
BGR, CD369, CANDF4, SCARE2, DECTIN1, CLECSF12
Gene ID
64581
Accession Number
NP_072092
Reactivity
Human
Target
Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology CLEC7A ELISA Kit (Human) (OKCA02090) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLEC7A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLEC7A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CLEC7A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
31.25 pg/mL-2,000 pg/mL
Recovery
Sample Type Mean (%) Range (%) Serum (n=5) 100 96-104 EDTA plasma (n=4) 96 90-102 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 100 96-104 Serum (n=5) 100 96-104 EDTA plasma (n=4) 96 90-102 EDTA plasma (n=4) 96 90-102
Sensitivity
7.81 pg/mL.
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
CLEC7A
Protein Name
C-type lectin domain family 7 member A
Gene Name URL
CLEC7A
Nucleotide Accession Number
NM_022570

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