SERPINE2 ELISA Kit (Mouse)

CAT: 0247-OKCA01676-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCA01676-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Serine (or cysteine) peptidase inhibitor, clade E, member 2
Gene Aliases
P, S, PN, ne, PI7, PI-7, PN-1, Spi4, PAI-1, B230326M24Rik
Gene ID
20720
Accession Number
NP_033281.1
Reactivity
Mouse
Target
Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Serpine2 ELISA Kit (Mouse) (OKCA01676) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpine2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Serpine2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Serpine2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
3.12 - 200 pg/mL
Recovery
Sample Mean Recovery (%) Range (%) Serum (n=5) 89 85-93 EDTA Plasma (n=4) 95 90-100 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 89 85-93 Serum (n=5) 89 85-93 EDTA Plasma (n=4) 95 90-100 EDTA Plasma (n=4) 95 90-100
Sensitivity
0.78 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
SERPINE2
Protein Name
Glia-derived nexin
Gene Name URL
SERPINE2
Nucleotide Accession Number
NM_009255.4

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