SERPINE2 ELISA Kit (Mouse)

• Gene Name :

  Serine (or cysteine) peptidase inhibitor, clade E, member 2

• Gene Aliases :

  P, S, PN, ne, PI7, PI-7, PN-1, Spi4, PAI-1, B230326M24Rik

• Gene ID :

  20720

• Accession Number :

  NP_033281.1

• Reactivity :

  Mouse

• Target :

  Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.

• Type :

  ELISA Kit

• Assay Principle :

  Aviva Systems Biology Serpine2 ELISA Kit (Mouse) (OKCA01676) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpine2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Serpine2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Serpine2 captured in the well.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Serum, Plasma, Tissue Homogenates

• Detection Range :

  3.12 - 200 pg/mL

• Recovery :

  Sample Mean Recovery (%) Range (%) Serum (n=5) 89 85-93 EDTA Plasma (n=4) 95 90-100 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 89 85-93 Serum (n=5) 89 85-93 EDTA Plasma (n=4) 95 90-100 EDTA Plasma (n=4) 95 90-100

• Sensitivity :

  0.78 pg/mL

• Reconstitution :

  Store as indicated in product manual.

• NCBI Gene Symbol :

  SERPINE2

• Protein Name :

  Glia-derived nexin

• Gene Name URL :

  SERPINE2

• CAS Number :

  7732-18-5

• Nucleotide Accession Number :

  NM_009255.4