Serpine2 ELISA Kit (Rat)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 413 2922 9164 595 3000 9451 Standard deviation 17.35 131.49 650.64 38.68 165.00 737.18 CV (%) 4.2 4.5 7.1 6.5 5.5 7.8 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 413 2922 9164 595 3000 9451 Mean (pg/ml) 413 2922 9164 595 3000 9451 Standard deviation 17.35 131.49 650.64 38.68 165.00 737.18 Standard deviation 17.35 131.49 650.64 38.68 165.00 737.18 CV (%) 4.2 4.5 7.1 6.5 5.5 7.8 CV (%) 4.2 4.5 7.1 6.5 5.5 7.8

• Gene Name:

  Serpin family E member 2

• Gene Aliases:

  Glia-derived nexin; GDN; Peptidase inhibitor 7; PI-7; Protease nexin 1; PN-1; Protease nexin I; Serpin E2; Serpine2; Pi7; Pn1

• Gene ID:

  29366

• Accession Number:

  NP_062070.1

• Reactivity:

  Rat

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: S20-P397

• Target:

  Glia-derived nexin is a protein that in humans is encoded by the SERPINE2 gene. This gene is mapped to 9q34. SERPINE2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 386 aminoacids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa) .SERPINE2 is expressed with a 8 aminoacid His tag atC-Terminus and purified by proprietary chromatographic techniques. This gene displays neurite promoting activity and serine protease inhibitory activity.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's rat SerpinE2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SerpinE2 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: S20-P397) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SerpinE2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates, serum and plasma (heparin, EDTA, citrate) and urine

• Detection Range:

  0.78 ng/ml - 50 ng/ml

• Sensitivity:

  <0.1 ng/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant rat SerpinE2

• NCBI Gene Symbol:

  SERPINE2

• Protein Name:

  Glia-derived nexin

• Gene Name URL:

  SERPINE2

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_019197.1