CD96 ELISA Kit (Human)

• Gene Name:

  CD96 molecule

• Gene Aliases:

  TACTILE

• Gene ID:

  10225

• Accession Number:

  NP_005807.1

• Reactivity:

  Human

• Target:

  May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.

• Type:

  ELISA Kit

• Assay Principle:

  Aviva Systems Biology CD96 ELISA Kit (Human) (OKCA01541) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD96 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD96 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CD96 captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates, Cell Lysates

• Detection Range:

  0.156 - 10 ng/mL

• Recovery:

  Sample Mean Recovery (%) Range (%) Serum (n=5) 89 84-93 EDTA Plasma (n=4) 93 88-97 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 89 84-93 Serum (n=5) 89 84-93 EDTA Plasma (n=4) 93 88-97 EDTA Plasma (n=4) 93 88-97

• Sensitivity:

  0.039 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• NCBI Gene Symbol:

  CD96

• Protein Name:

  T-cell surface protein tactile

• Gene Name URL:

  CD96

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_005816.4