CD99 ELISA Kit (Human)

CAT: 0247-OKCD01585-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD01585-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cluster Of Differentiation 99 (CD99) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cluster Of Differentiation 99 (CD99) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
CD99 molecule (Xg blood group)
Gene Aliases
12E7; antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13; CD99 antigen; cell surface antigen 12E7; cell surface antigen HBA-71; cell surface antigen O13; E2 antigen; HBA71; MIC2; MIC2 (monoclonal antibody 12E7) ; MIC2X; MIC2Y; MSK5X; Protein MIC2; surface antigen MIC2; T-cell surface glycoprotein E2.
Gene ID
4267
Accession Number
NP_001116370.1
Reactivity
Homo sapiens|Human
Target
Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 99 (CD99) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 99 (CD99) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 99 (CD99), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 99 (CD99) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery Range (%) Average (%) _x005F Serum (n=5) 82-105 86 _x005F EDTA Plasma (n=5) 80-91 87 _x005F Heparin Plasma (n=5) 80-103 91 _x005F Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum (n=5) 82-105 86 Serum (n=5) 82-105 86 EDTA Plasma (n=5) 80-91 87 EDTA Plasma (n=5) 80-91 87 Heparin Plasma (n=5) 80-103 91 Heparin Plasma (n=5) 80-103 91
Sensitivity
< 0.065 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount _x005F Anti-CD99 Microplate 96 Wells (12 x 8 Well Strips) _x005F CD99 Lyophilized Standard 2 x 10 ng _x005F 100X Biotinylated CD99 Detector Antibody 120 uL _x005F Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount Component Amount Anti-CD99 Microplate 96 Wells (12 x 8 Well Strips) Anti-CD99 Microplate 96 Wells (12 x 8 Well Strips) CD99 Lyophilized Standard 2 x 10 ng CD99 Lyophilized Standard 2 x 10 ng 100X Biotinylated CD99 Detector Antibody 120 uL 100X Biotinylated CD99 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBKM4
Specificity
This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 99 (CD99) ._x005F No significant cross-reactivity or interference between Cluster Of Differentiation 99 (CD99) and analogues was observed.
Protein Length
185
NCBI Gene Symbol
CD99
Protein Name
CD99 antigen
Gene Name URL
CD99
Nucleotide Accession Number
NM_001122898.2

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