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NPY ELISA Kit (Rat)

CAT: 0247-OKCA00987-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00987-96WSize:96 Well
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Neuropeptide Y
Accession Number
NP_001153758.1
Reactivity
Rat
Target
NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology NPY ELISA Kit (Rat) (OKCA00987) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for NPY has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPY is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample NPY captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
1.56 pg/mL-100 pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 93 87-99 EDTA Plasma (n=4) 105 100-110 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 93 87-99 Serum (n=5) 93 87-99 EDTA Plasma (n=4) 105 100-110 EDTA Plasma (n=4) 105 100-110
Sensitivity
0.39 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
NPY
Protein Name
Neuropeptide Y
Gene Name URL
NPY
Nucleotide Accession Number
NM_001160286.1

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