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ARG1 ELISA Kit (Rat)

CAT: 0247-OKCA00948-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00948-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Arginase-1
Gene Aliases
Arginase-1, Liver-type arginase, Type I arginase
Gene ID
100008814
Accession Number
NP_001075577.1
Reactivity
Rat
Target
This protein is involved in step 1 of the subpathway that synthesizes L-ornithine and urea from L-arginine.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology ARG1 ELISA Kit (Rat) (OKCA00948) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for ARG1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ARG1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ARG1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 104 95-109 EDTA Plasma (n=4) 101 91-105 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 104 95-109 Serum (n=5) 104 95-109 EDTA Plasma (n=4) 101 91-105 EDTA Plasma (n=4) 101 91-105
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
ARG1
Protein Name
Arginase-1
Gene Name URL
ARG1
Nucleotide Accession Number
NM_001082108.1

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