PRCP ELISA Kit (Mouse)

Inter-Assay CV:

7732-18-5

Prolylcarboxypeptidase (angiotensinase C)

PCP, HUMPCP, AI451719, 2510048K03Rik, 2610104A14Rik

72461

NP_082519.1

Mouse

Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.

ELISA Kit

Aviva Systems Biology Prcp ELISA Kit (Mouse) (OKCA00905) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Prcp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Prcp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Prcp captured in well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue Homogenates And Cell Lysates

37.5 pg/mL-2,400 pg/mL

Matrix Recovery range (%) Average (%) Serum (n=5) 92 87-98 EDTA Plasma (n=4) 94 93-104 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 92 87-98 Serum (n=5) 92 87-98 EDTA Plasma (n=4) 94 93-104 EDTA Plasma (n=4) 94 93-104

9.37 pg/mL

Store as indicated in product manual.

PRCP

Lysosomal Pro-X carboxypeptidase

PRCP